Comparison of the gut microbiota and untargeted gut tissue metabolome of Chinese mitten crabs (Eriocheir sinensis) with different shell colors
نویسندگان
چکیده
Introduction The Chinese mitten crab ( Eriocheir sinensis ) is a highly valued freshwater crustacean in China. While the natural shell color of E. greenish brown (GH), we found variety with brownish-orange (RH). Although RH more expensive, it exhibits lower molting frequency and growth rate compared GH, which significantly reduces its yield hinders large-scale farming. development animals are closely related to their gut microbiota tissue metabolic profiles. Methods In this study, microbiome communities profiles juvenile GH crabs using 16S rRNA gene sequencing liquid chromatography–mass spectrometry (LC–MS), respectively. Results Our findings indicated that intestinal microbial composition characteristics differed between GH. At operational taxonomic unit (OTU) level, α-diversity did not differ while β-diversity was higher than microbiota. species richness unclassified_c_Alphaproteobacteria group, group had three low-abundance species, Flavobacteria bacterium BAL38 , Paraburkholderia ferrariae uncultured_bacterium_g__Legionella . current 598 metabolites were identified, 159 different RH. metabolite profile characteristic low level most amino acids lipid high several pigments These enriched 102 KEGG pathways. Four pathways, including (1) Central carbon metabolism cancer, (2) protein digestion absorption, (3) alanine, aspartate glutamate metabolism, (4) aminoacyl-tRNA biosynthesis, enriched. correlation analysis microbiotas key differential positively correlated abundance Shewanella _sp_MR-7. Discussion This research provided greater understanding physiological conditions varieties colors by comparing metabolome.
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ژورنال
عنوان ژورنال: Frontiers in Microbiology
سال: 2023
ISSN: ['1664-302X']
DOI: https://doi.org/10.3389/fmicb.2023.1218152